2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

Related Products

PDF 1.61 MB

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-158672
    3′-O-Azidomethyl-dTTP sodium
    3′-O-Azidomethyl-dTTP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    3′-O-Azidomethyl-dTTP sodium
  • HY-160813
    DHX9-IN-18
    		Inhibitor
    DHX9-IN-13 (Example 1) is a RNA helicase DHX9 inhibitor with an EC50 of 16.4 μM. DHX9-IN-13 exhibits biochemical infection point of 0.32 μM. DHX9-IN-13 can be used for the study of cancers.
    DHX9-IN-18
  • HY-151292
    Antitumor agent-74
    		Inhibitor
    Antitumor agent-74 (compound 13da) is a quinoxalines derivative, an antitumor agent. Antitumor agent-74 exhibits more potent efficacy on tumor inhibition, mixed with regioisomer Antitumor agent-75(HY-151295, compound 14 da) (mriBIQ 13da/14da). mriBIQ 13da/14da attests cell cycle at S phase, inhibits DNA synthesis, and induces mithochondrial apoptosis.
    Antitumor agent-74
  • HY-W048482S3
    rU Phosphoramidite-15N2
    rU Phosphoramidite-15N2 (DMT-2'O-TBDMS-rU phosphoramidite-15N2) is 15N labeled rU Phosphoramidite (HY-W048482). rU Phosphoramidite is a phosphorite monomer that can be used in the synthesis of oligonucleotides.
    rU Phosphoramidite-<sup>15</sup>N<sub>2</sub>
  • HY-158580
    5-CF3-ddUTP sodium
    5-CF3-ddUTP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    5-CF3-ddUTP sodium
  • HY-147988
    DNA Gyrase-IN-5
    		Inhibitor
    DNA Gyrase-IN-5 (Compound 8I-w) is a potent DNA gyrase inhibitor with an IC50 of 0.10 μM. DNA Gyrase-IN-5 shows antibacterial activities against wild type and drug-resistant strains.
    DNA Gyrase-IN-5
  • HY-137611A
    α,β-MTDP trisodium
    		Inhibitor
    α,β-MTDP trisodium, a thymidylate analog, is a thymidine kinase inhibitor with a Ki of 23 µM.
    α,β-MTDP trisodium
  • HY-134991
    3'-Deoxy-GTP
    		Inhibitor
    3'-Deoxy-GTP (3′-Deoxyguanosine 5′-triphosphate), an analog of GTP, is a RNA chain terminator and suppresses RNA synthesis. 3'-Deoxy-GTP inhibits DENV NS5 RdRp (IC50: 0.02 μM).
    3'-Deoxy-GTP
  • HY-146317
    P1788
    		Inhibitor
    P1788 is a dihydroorotate dehydrogenase (DHODH) inhibitor. P1788 induces DNA damage.
    P1788
  • HY-160841
    Biotin-16-dCTP
    Biotin-16-dCTP is a dNTP with a high incorporation rate that can be used to optimize dNTP DNA polymerase complexes for target nucleic acid amplification.
    Biotin-16-dCTP
  • HY-W744235
    Levofloxacin-d3 sodium
    Levofloxacin-d3 sodium is the deuterium labeled Levofloxacin sodium (HY-B0330C). Levofloxacin ((-)-Ofloxacin) sodium is an orally active antibiotic and is active against both Gram-positive and Gram-negative bacteria. Levofloxacin sodium inhibits the DNA gyrase and topoisomerase IV. Levofloxacin sodium can be used for chronic periodontitis, airway inflammation and BK Viremia research. Levofloxacin sodium shows anti-orthopoxvirus activity.
    Levofloxacin-d<sub>3</sub> sodium
  • HY-132267S
    N-Nitrosodibenzylamine-d4
    		Inhibitor
    N-Nitrosodibenzylamine-d4 is deuterium labeled N-Nitrosodibenzylamine. N-Nitrosodibenzylamine is a potent and orally activity DNA damage inducer. N-Nitrosodibenzylamine induces DNA single-strand breaks (SSBs).
    N-Nitrosodibenzylamine-d<sub>4</sub>
  • HY-13550
    Ametantrone
    		Inhibitor
    Ametantrone (NSC 196473) is an antitumor agent that intercalates into DNA and induces topoisomerase II (TOP2)-mediated DNA break.
    Ametantrone
  • HY-N6712R
    Thiolutin (Standard)
    		Inhibitor
    Thromycin (Standard) is the analytical standard of thromycin (Acetopyrrothin) (HY-N6712). Thiolutin is a sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. Thiolutin can be produced by Streptomyces. Thiolutin inhibits AMSH (IC50 = 4 μM) and Rpn11 (IC50 = 0.53 μM). Thiolutin is a dual inhibitor of BRCC36 and the NLRP3 inflammasome, exhibiting anti-inflammatory effects. Thiolutin effectively suppresses the interaction between BRCC36 and HMGCR, leading to the inhibition of HCC growth. Thiolutin attenuates pyroptosis and NLRP3 inflammasome activation. Thiolutin markedly alleviates renal injury and inflammatory process in IgAN. Thiolutin is an anti-angiogenic compound which can ease Doxorubicin (HY-15142A)-induced cardiotoxicity (DOXIC)[1][2][3][4][5].
    Thiolutin (Standard)
  • HY-162146
    NS3-IN-1
    		Inhibitor
    NS3-IN-1 (compound 302) is a potent helicase (NS3) inhibitor. NS3-IN-1 shows an potent inhibitor for yellow fever helicase.
    NS3-IN-1
  • HY-126824
    5,10-Dideazaaminopterin
    5,10-Dideazaaminopterin (compound 1) is a new compound with activity against L1210 leukemia with an IC50 value of 17nM.
    5,10-Dideazaaminopterin
  • HY-137658A
    Purine riboside-5'-O-triphosphate sodium
    		Inhibitor
    Purine riboside-5'-O-triphosphate sodium, an active metabolite of Nebularine (HY-103694), acts as an inhibitor of DNA primase ATP and GTP polymerization activities, with IC50 values of 35 µM and 28 µM for the human enzyme, respectively. Purine riboside-5'-O-triphosphate sodium inhibits calmodulin-dependent protein kinase II (CaMKII) with a Ki value of 590 µM.
    Purine riboside-5'-O-triphosphate sodium
  • HY-16445BR
    CNDAC hydrochloride (Standard)
    		Inhibitor
    Dioscin (Standard) is the analytical standard of Dioscin. This product is intended for research and analytical applications. Dioscin (CCRIS 4123; Collettiside III) is a natural plant-derived steroidal saponin that has good anti-cancer activity against a variety of cancer cells. Dioscin causes DNA damage and induces apoptosis in HeLa and SiHa cells. Dioscin regulates ROS-mediated DNA damage and mitochondrial signaling pathways, exerting anticancer activity.
    CNDAC hydrochloride (Standard)
  • HY-W015490R
    1,4-Naphthoquinone (Standard)
    		Inhibitor
    1,4-Naphthoquinone is an inhibitor with broad-spectrum inhibitory activity targeting DNA polymerase, NF-κB and monoamine oxidase (MAO-A/B), with antibacterial and anti-biofilm efficacy. 1,4-Naphthoquinone is a competitive inhibitor of MAO-B (Ki=1.4 μM) and a non-competitive inhibitor of MAO-A (Ki=7.7 μM). 1,4-Naphthoquinone inhibits DNA polymerase pol α, β, γ, δ, ε, λ with IC50 ranging from 5.57-128 μM. 1,4-Naphthoquinone inhibits tumor cell proliferation, induces apoptosis and necrosis, and has anti-angiogenic and anti-inflammatory activities by inducing oxidative stress, depleting glutathione (GSH), inhibiting DNA polymerase-mediated DNA synthesis and blocking NF-κB nuclear translocation. 1,4-Naphthoquinone can be used in anti-bacterial , anti-tumor and anti-inflammatory studies, including inhibition of melanoma and colon cancer cell growth and endothelial cell function, as well as LPS-induced inflammation models.
    1,4-Naphthoquinone (Standard)
  • HY-W141393
    2'-OMe-dmf-G-CE-Phosphoramidite
    2'-OMe-dmf-G-CE-Phosphoramidite is a phosphorite monomer that can be used in the synthesis of oligonucleotides.
    2'-OMe-dmf-G-CE-Phosphoramidite
Cat. No. Product Name / Synonyms Application Reactivity